Publication: Antigens from saliva as markers of canine exposure to visceral leishmaniases vector

Researchers have identified three salivary proteins which could be used individually or in combination for screening canine sera in endemic areas of visceral leishmaniases for vector exposure and for estimating the risk of Leishmania infantum transmission in dogs.

Writing in PLOS Neglected Tropical Diseases, they report: “The protozoan parasite Leishmania infantum is a causative agent of zoonotic visceral leishmaniasis, an important and potentially fatal human disease. The main reservoir hosts of this Leishmania species are dogs, and the only proven vectors are phlebotominae sand flies, Phlebotomus perniciosus being considered the major vector in the western Mediterranean area.

“During feeding on the host, sand flies spit saliva into the host skin; hosts develop a specific antibody response directed against sand fly salivary proteins and levels of these antibodies reflect the intensity of sand fly exposure. As the availability of salivary gland homogenate is limited, recombinant salivary proteins have been suggested as antigens suitable for measuring specific antibody levels.

“In the present work, we expressed six of the most-antigenic salivary proteins, and studied the mice and canine humoral immune responses to these recombinant proteins. We demonstrated that three proteins, a yellow-related protein and two apyrases, are suitable antigens for measuring anti-P. perniciosus antibody levels and estimating the host exposure to this sand fly species.”

Taken from: Drahota J, Martin-Martin I, Sumova P, Rohousova I, Jimenez M, Molina R, et al. (2014) Recombinant Antigens from Phlebotomus perniciosus Saliva as Markers of Canine Exposure to Visceral Leishmaniases Vector. PLOS Neglected Tropical Diseases 8(1): e2597. Doi:10.1371/journal.pntd.0002597 (EDENext152)

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Published: 06/05/2015

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